High-performance liquid chromatography (HPLC) is a common technique used for the analysis of pharmaceutical drugs and impurities, chemicals in food, flavors, oils and fuels, polymers, and more. HPLC works by separating different types of molecules in specialized flow-through columns. The separated molecules are subsequently identified and/or quantified using a myriad of detectors. Common detectors include those based on ionization (e.g., mass spectrometry), light absorption (e.g., ultraviolet-visible spectroscopy), light refraction (e.g., refractive index), and light scattering (e.g., evaporative light scattering or charged aerosol).

Therapeutic proteins are used to treat a wide variety of diseases, from cancer and heart disease to cystic fibrosis and diabetes. These proteins currently are produced using microbial fermentation in cell cultures and by transgenic animals or plants. They represent a nearly $200 billion market, yet product stability and unintended immune responses remain significant obstacles to treatment. For example, many therapeutics suffer from short half-lives that require frequent dosing, which can lead to increased side effects and noncompliance to treatment. Many other therapies produce immune reactions that impair therapeutic activity or endanger the patient. Often, these challenges arise from a limitation inherent to all proteins: they are made from the same 20 amino acid building blocks.

Poor air quality has serious public health consequences, as highlighted recently by the COVID-19 pandemic and Western U.S. wildfires. According to World Health Organization (WHO) and World Bank reports, the annual societal cost associated with air pollution is close to eight million premature deaths and $5.7 trillion.

Our agricultural system is facing tremendous challenges in sustainably meeting the food demands of the growing world population. An evaluation of the crop protection sector found that the total cost of using chemical pesticides — including regulatory, human health, environmental, and exposure-prevention costs — exceeds $39.5 billion per year in the U.S. (1).

The world is not on track to achieve the United Nations (UN) sustainable development goal of clean water for all by 2030. Population growth, urbanization, and climate change aggravate water scarcity. The current devastating drought in the western U.S. is a result of these factors coupled with long-term over-withdrawals of freshwater resources. To meet potable water needs, communities are turning to non-freshwater sources. Today’s best technological solution to purify non-freshwater sources into potable water is reverse osmosis (RO), the most cost-effective and energy-efficient method of desalination.